Bacterial diversity in the oral cavity of adolescents with different caries susceptibilities.
- Author:
Yangyang ZHANG
;
Jinzhi HE
;
Xin XU
;
Xuedong ZHOU
- Publication Type:Journal Article
- MeSH: Adolescent; Bacteria; Child; DNA, Bacterial; analysis; Denaturing Gradient Gel Electrophoresis; Dental Caries; microbiology; Dental Caries Susceptibility; Humans; Microbiota; Mouth; microbiology; Polymerase Chain Reaction; RNA, Ribosomal, 16S; Saliva; microbiology
- From: West China Journal of Stomatology 2015;33(6):602-606
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the differences between the bacterial diversities in the saliva of caries-free and caries-susceptible adolescents through polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE).
METHODSTwenty adolescent subjects aged 12-18 years were recruited and subdivided into two groups: caries-free adolescents (n = 10) and caries-susceptible adolescents (n = 10). Saliva samples were collected. Total DNA was isolated directly from each sample. A portion of the 16S rRNA gene locus was PCR-amplified by using universal primers. Microbial diversity was analyzed through PCR-DGGE.
RESULTSAnalyzing the DGGE profile, we found that the composition of the saliva microbiome exhibited great intra-individual differences; the average band numbers of the caries-free adolescent group and the caries-susceptible adolescent group were 32.5 ± 3.7 and 27.3 ± 3.4, respectively. The differences between the groups were statistically significant (P = 0.008). Shannon-Wiener's indexes of the caries-susceptible adolescent group and the caries-free adolescent group were 2.5 ± 0.2 and 2.6 ± 0.2, respectively, but the differences between the groups were not significant (P = 0.405). Clustering analysis results suggested that most of the samples in the same group clustered together; this observation showed a high community structure similarity.
CONCLUSIONThe microbial diversity and complexity of bacteria in saliva are significantly higher in caries-free adolescents than in caries-susceptible adolescents. During caries development, bacterial diversity in the saliva likely decreases.