Expression of heat shock protein 27 in cigarette smoke extract-induced injury of human gingival fibroblasts.

Author: Qin ZHOU ; Ruinan SUN ; Zhenkun ZHU
Publication Type:Journal Article
MeSH: Cells, Cultured; Fibroblasts; Gingiva; HSP27 Heat-Shock Proteins; Humans; Smoke; Tobacco; Wound Healing
From: West China Journal of Stomatology 2016;34(1):17-22
CountryChina
Language:Chinese
Abstract:
OBJECTIVEThis research aimed to observe the expression of heat shock protein 27 (HSP27) in human gingival fibroblasts (HGFs) cell injury induced by different concentrations of cigarette smoke extract (CSE).
METHODSThe third to eighth generations of cultured HGFs were treated with serially diluted CSE of different concentrations (0, 2.5%, 5.0%, 12.5%, 25.0%, 50.0%). Wound-healing assay was performed to determine the migration of HGFs, and Western blot was used to determine the expression of HSP27.
RESULTSThe migration capability of HGFs weakened with the increase of CSE concentration. HSP27 expression was negative in normal HGFs but positive in CSE-intervened HGFs in a concentration-dependent manner.
CONCLUSIONHSP27 concentration increased in the CSE-induced injury of HGFs. This finding suggests that HSP27 plays an important role in CSE-induced epithelial injury.