Effects of different frequency microvibrations in the vascular endothelial growth factor expression and permeability of vascular endothelial cell.
- Author:
Zhuoli ZHU
;
Ruiyang MA
;
Yang YANG
;
Xueqi GAN
- Publication Type:Journal Article
- MeSH: Blotting, Western; Capillary Permeability; physiology; Endothelial Cells; metabolism; Endothelium, Vascular; chemistry; Humans; Permeability; Polymerase Chain Reaction; RNA, Messenger; analysis; Umbilical Cord; chemistry; metabolism; Up-Regulation; Vascular Endothelial Growth Factor A; analysis; genetics; physiology
- From: West China Journal of Stomatology 2016;34(2):136-139
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThis study aimed to evaluate the vascular endothelial growth factor (VEGF) expression and permeability of vascular endothelial cell under microvibration.
METHODSHuman umbilical vein endothelial cell (HUVEC) were cultured, randomly vibrated under low frequency of 0.2, 0.5, 2, 5 Hz, 30 min per day. The VEGF mRNA level was detected by Tagman probe real-time fluorescence quantitative polymerase chain reaction (PCR), and the VEGF protein expression level was detected by Western blot. The permeability of vascular endothelial cell was evaluated.
RESULTSCompared with the blank control group, the mRNA and protein expression level of VEGF were significantly increased under 0.2, 0.5 Hz thelial, and increase the permeability microvibration (P<0.05), and decreased under 2, 5 Hz microvibration (P<0.01). The vascular endothelial permeability in creased under 0.2, 0.5 Hz microvibration (P<0.01), whereas the permeability decreased under 2, 5 Hz microvibration (P<0.01).
CONCLUSION0.2-0.5 Hz microvibration can up-regulate the expression of VEGF mRNA and protein in vascular endothelial, and increase the permeability.