Nuclear factor-κB pathway mediates the effects of CD137 signaling on NFATc1 expression in mice vascular smooth muscle cells
10.3760/cma.j.issn.0253-3758.2015.07.010
- VernacularTitle:CD137信号通过核因子κB影响小鼠主动脉平滑肌细胞活化T细胞核因子c1表达
- Author:
Yunjie YIN
1
;
Jinchuan YAN
;
Zhongqun WANG
;
Peijing LIU
;
Yi LIANG
Author Information
1. 江苏大学附属医院心内科
- Keywords:
Atherosclerosis;
NF-kappa B;
NFATC transcription factors
- From:
Chinese Journal of Cardiology
2015;43(7):614-618
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe whether CD137 signaling could affect the nuclear factor of activated T cells c1 (NFATc1) expression through nuclear factor-κB (NF-KB) pathway in mice aortic vascular smooth muscle cells (VSMCs).Methods Adherence methods for tissues explants were used for primary culture of mouse aortic VSMCs.The mRNA expression of CD137 and NFATc1 was detected by realtime quantitative PCR (RT-qPCR).The VSMCs protein expression of IκB-α,NF-κB p65,phospo-p65 and NFATc1 was determined by Western blot.The level of CD137 was measured by Flow Cytometry (FCM).Results (1)The mRNA and protein expression of CD137 in VSMCs was significantly upregulated at 24 h after co-culture with TNF-α (10 ng/ml,all P ＜ 0.05).(2) Compared with the control group,the level of p-NF-κB p65 in cytoplasm and nucleus was significantly increased (8.34 ± 0.28 vs.1,P ＜ 0.05,and 2.64 ± 0.42 vs.1,P ＜ 0.05) while the level of IκB-α was reduced (1 vs.2.70 ± 0.28,P ＜ 0.05) after co-treatment with agonist-CD137 mAb,above effects were partly blocked by adding specific NF-κB inhibitor PDTC (30 μmol/L:1.15 ±0.14 vs.8.34 ±0.28,P ＜0.05,and 2.09 ±0.12 vs.2.64 ±0.42,P ＜0.05,and1.78 ± 0.74 vs.1,P＜0.05).(3)The mRNA (2.07±0.09 vs.1,P＜0.05) and protein (1.75 ± 0.07 vs.1,P ＜ 0.05) expression of NFATc1 was significantly upregulated by agonist CD137mAb compared with the control group,and these effects could be reversed by PDTC (1.15 ±0.07 vs.2.07 ±0.09,P ＜ 0.05,and 0.90 ±±0.11 vs.1.75 ±0.07,P＜0.05).Conclusion CD137 signaling could affect the NFATc1 expression in VSMCs through NF-kappaB pathway.
