The Interaction of Methylenetetrahydrofolate Reductase Polymorphism with Homocysteine in Pregnant Women.
- Author:
Young Ju KIM
1
;
Jong Soon PARK
;
Mi Hye PARK
;
Namsoo CHANG
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, Ewha Womans' University, Korea.
- Publication Type:Original Article
- Keywords:
Homocysteine;
Vitamin B12;
Folic acid;
MTHFR mutation
- MeSH:
Chromatography, Liquid;
DNA;
Female;
Folic Acid;
Genotype;
Homocysteine*;
Humans;
Methylenetetrahydrofolate Reductase (NADPH2)*;
Oxidoreductases;
Polymerase Chain Reaction;
Pregnant Women*;
Vitamin B 12;
Vitamins
- From:Korean Journal of Obstetrics and Gynecology
2003;46(8):1525-1530
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: The purpose of this study was to investigate the relationship between serum levels of folic acid, vitamin B12 and homocysteine and C677T metylenetetrahydrofolate reductase (MTHFR) mutation in pregnant women. METHODS: DNA was extracted from whole blood of 177 pregnant women. All samples were genotyped for the C677T polymorphism in MTHFR gene by polymerase chain reaction (PCR-RELP). Serum levels of homocysteine, folate, and vitamin B12 were measured by high performance liquid chromatography for homocysteine, and radioassay for folate and vitamin B12. RESULTS: Serum homocysteine was higher in women with the T/T genotype than those with the C/T or the C/C genotype of the MTHFR gene (p<0.05). Serum homocysteine was negatively correlated with serum folate in all MTHFR genotypes (p<0.001). Serum homocysteine was increased in pregnant women with the T/T type of the MTHFR gene only when the serum folate fell below the median compared to those stayed above the median level (p<0.05). Serum folate was positively correlated with serum vitamin B12 in all subjects. CONCLUSION: Serum homocysteine varied significantly by the MTHFR genotype and the serum B vitamin status.
