Exercise preconditioning attenuates pressure overload-induced pathological cardiac hypertrophy: potential role of HSF1 and NF-κB p65 signaling
10.3760/cma.j.issn.0253-3758.2015.10.011
- VernacularTitle:热休克转录因子1和核转录因子κB p65参与运动预处理减轻压力超负荷诱导大鼠病理性心肌肥厚的机制
- Author:
Tongyi XU
1
;
Tao LI
;
Fan YANG
;
Qingqi HAN
;
Liangjian ZOU
Author Information
1. 解放军第四○一医院胸心外科
- Keywords:
Cardiomegaly;
Exercise therapy;
NF-kappa B;
HSF1
- From:
Chinese Journal of Cardiology
2015;43(10):894-899
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of exercise preconditioning (EP) on pressure overload-induced pathological cardiac hypertrophy and explore related mechanisms.Methods Ten-week-old male Sprague-Dawley rats (n =80) were randomly divided into four groups via random number table method: sham, TAC,EP + sham and EP + TAC.Two EP groups were subjected to 4 weeks of treadmill training, and followed by sham and TAC operations.Eight weeks after the surgery, mean arterial pressure (MAP), cardiac morphology, mRNA expressions of the B-type natriuretic peptide (BNP) and heat shock protein (HSP) 70 and protein expression of the BNP, heat shock transcription factor 1 (HSF1), HSP70, nuclear factor κB (NF-κB) p65, and interleukin-2 (IL-2) were examined.Results (1) Pathological cardiac hypertrophy index: eight weeks after TAC, MAP, heart size, HW/BW, cross-sectional area of the cardiomyocytes (CSA) and mRNA and protein expressions of BNP in the LV were all significantly higher in the TAC and EP + TAC groups than respective sham groups (all P < 0.05).HW/BW, CSA, and mRNA and protein expressions of BNP in the LV were significantly lower in EP + TAC group than in TAC group (all P <0.05).(2) mRNA and protein expressions of HSF1 and HSP70 and nuclear HSF1 levels were significantly downregulated post TAC, however, EP treatment significantly increased the expression of HSFl and nuclear HSF1 levels in TAC rats (all P < 0.05).(3) mRNA and protein expressions of NF-κB p65 and IL-2 were significantly increased in the TAC and EP + TAC groups compared with the respective sham groups (all P < 0.05), which were significantly downregulated in EP + TAC group compared to TAC group (all P < 0.05).Conclusions EP could effectively reduce the cardiac hypertrophic responses induced by TAC possibly through upregulating the expressions of HSF1 and HSP70 and inhibiting the expression of NF-κB p65 and its nuclear translocation.