Effect of DRB on the Biological Characteristics of Human Laryngeal Carcinoma Hep-2 Cell Line
- Author:
Jianting WANG
1
;
Shusheng GONG
;
Yong FU
;
Qiuhong XUE
;
Guangli CHEN
;
Yingpeng LIU
Author Information
1. 华中科技大学同济医学院附属协和医院
- Keywords:
protein-serine-threonine kinases;
5,6-Dichloro-1-β-D-ribofuranosyl- benzimidazole;
laryngeal neoplasms;
Hep-2 cell line
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2007;27(1):104-106
- CountryChina
- Language:Chinese
-
Abstract:
In order to study the effect of 5, 6-Dichloro-1-β-D-ribofuranosyl-benzimidazole (DRB) on the biological characteristics of human laryngeal carcinoma Hep-2 cell line in vitro, Hep-2 cells cultured in vitro were treated with different concentrations of DRB. Changes in cell proliferation, apoptotic rate and invasiveness were detected by MTT assay, flow cytometry (FCM) and matrigel in vitro invasion assay, respectively. It was found that DRB inhibited the proliferation of Hep-2 cells in a dose- and time-dependent manner. After being treated with 0, 10, 20, 40, 80 μmmol/L DRB for 24 h, the apoptotic rate in Hep-2 cells was (0.68±0.19) %, (1.95±0.12)%, (8.51 ±0.26)%, (11.26±0.17) % and (14.99±0.32)%, respectively. The matrigel in vitro invasion assay revealed that DRB began to inhibit the invasion of Hep-2 cells at the concentration of 5 μmmol/L, and with the increase of DRB concentration, the inhibitory effect was enhanced. It was suggested that DRB could influence the essential biological characteristics of Hep-2 cells, inhibit Hep-2 cells proliferation, reduce invasive ability and induce apoptosis of Hep-2 cells.
