Irradiation Injury Temporarily Induces Enhancement of APN/CD13 Peptidase Activity on Aorta-gonads-mesonephros (AGM)-derived Stromal Cells
- Author:
Yan ZHU
1
;
Lifang HUANG
;
Xiaohua LUO
;
Hanying SUN
;
Dan RAN
;
Kejie ZHANG
;
Miao ZHENG
;
Kun ZHOU
;
Wenli LIU
Author Information
1. Tongji Hospital, Tongji Medical School, Huazhong University of Science and Technology
- Keywords:
APN/CD13;
aorta-gonads-mesonephros (AGM) region;
stromal cell;
irradiation injury;
enzymatic activity
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2007;27(2):145-148
- CountryChina
- Language:Chinese
-
Abstract:
This study was designed to investigate the expression of aminopeptidase N (APN)/CD13 on intraembryonic AGM stromal cells, and the change of its enzymatic activity after irradiation injury.The expression of APN/CD13 on AGM stromal cells was assayed by RT-PCR and immunihistochemistry. After the stromal cells in AGM region were irradiated with 8.0 Gy of 60Co γ-rays, APN/CD13 enzymatic activity was measured by spectrophotometer at different time points. The result showed that AGM stromal cells strongly expressed APN/CD13. The enzymatic activity of APN/CD13 decreased temporarily after irradiation injury, then increased to higher level 4 h after irradiation, and it returned to the pre-irradiation level 24 to 48 h after the irradiation. The enzymatic activity of APN/CD13 was temporarily enhanced after irradiation injury, which might be one of the compensatory mechanisms that promote the hematopoietic recovery after irradiation.
