Establishment of a Simple and Useful Way for Preimplantation Genetic Diagnosis of Chromosomal Diseases
- Author:
Haining LUO
1
;
Guijin ZHU
;
Qun LIU
;
Wen CHEN
;
Zhou LI
Author Information
1. 华中科技大学同济医学院附属同济医院
- Keywords:
biopsy;
fixation;
fluorescence in situ hybridization;
preimplantation genetic diagnosis
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2007;27(3):315-317
- CountryChina
- Language:Chinese
-
Abstract:
In order to establish a simple and useful way for preimplantation genetic diagnosis (PGD)of chromosomal diseases in general IVF laboratory, the methods that are most commonly used in the embryo biopsy, fixation of blastomere and fluorescence in situ hybridization were compared. The three aspects of PGD were analyzed respectively. There was no significant difference in further development capacity of embryos between mechanical (79.7%) and chemical biopsy group (78.6%)(P＞0.05). In this study, more cells were successfully fixed with the Tween/HCL method (93.8%) than with the methanol/acetic acid method (80.5%, P＜0.05). There was no significant difference in cytoplasm remains between methanol/acetic acid method and Tween/HCL method (P＞0.05). The hybridization efficiency of fluorescence in situ hybridization was 89.5% in successive denaturation method and 90.9% in codenaturation method with the difference being not significant (P＞0.05). In conclusion, the mechanical or chemical method, Tween/HCL fixation method and codenaturation fluorescence in situ hybridization method can constitute a simple and useful way for PGD of chromosomal diseases.
