Effect of tacrolimus on growth-associated protein-43 expression in the hippocampus of neonatal rats with hypoxic-ischemic brain damage.
- Author:
Yan ZHOU
1
;
Ying XIONG
;
San-Ying YUAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; GAP-43 Protein; analysis; Hippocampus; chemistry; drug effects; Hypoxia-Ischemia, Brain; drug therapy; metabolism; pathology; Immunosuppressive Agents; pharmacology; Rats; Rats, Sprague-Dawley; Tacrolimus; pharmacology
- From: Chinese Journal of Contemporary Pediatrics 2009;11(1):65-68
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEImmunosuppressant tacrolimus (FK506) has shown neuroprotective effects on hypoxic-ischemic brain damage (HIBD) in the adult animal model. This study investigated whether FK506 has a protection against HIBD in neonatal rats by examining growthjassociated protein-43 (GAP-43) expression in the hippocampus.
METHODSNinety-six seven-day-old Sprague-Dawley rats were randomly divided into three groups: sham-operation, HIBD and FK506 intervention group. HIBD was induced in the later two groups. The FK506 intervention group was intraperitoneally injected with FK506 immediately after HIBD, at a dosage of 1 mg/kg daily, for three days. The HIBD group was injected with normal saline. Immunohistochemical technical was applied to examine GAP-43 expression in the hippocampus 24 and 72 hrs and 7 and 14 days after HIBD.
RESULTSCompared with the HIBD group, hematoxylin-eosin staining showed attenuated neuronal necrosis in the FK506 intervention group. In the HIBD group, the expression of GAP-43 increased significantly 72 hrs, and 7 and 14 days after HIBD compared with that in the sham-operation group. The GAP-43 expression in the FK506 intervention group was significantly higher than that in the HIBD group 72 hrs and 7 days after HIBD.
CONCLUSIONSFK506 might have neuroprotective effects against HIBD in neonatal rats.
