Induced RAS association domain family gene 1A gene expression by arsenic trioxide in nasopharyngeal carcinoma cell
10.3760/cma.j.issn.1673-0860.2009.10.019
- VernacularTitle:亚砷酸诱导人鼻咽癌细胞Ras相关结构域家族1A基因去甲基化表达的研究
- Author:
Jun-Li HU
1
;
Yue-Fei ZHANG
;
Zhi-Dong QIU
;
Hai-Hui LIANG
Author Information
1. 广东医学院附属医院
- Keywords:
Nasophatyngeal neoplasms;
Arsenicals;
Oxides;
Methylation;
Tumor suppressor proteins
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2009;44(10):866-870
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of arsenic trioxide (As_2O_3) on expression of anti-oncogene RAS association domain family gene 1A(RASSF1A) in nasopharyngeal carcinoma cell line CNE-2Z. Methods CNE-2Z cells were treated with various concentrations of As_2O_3 for different times. The IC_(50) values were detected by trypan blue stain assay. Cell cycle redistribution was analyzed by flow cytometry. The final concentration 2 μmol/L,1 μmol/L,0. 5 μmol/L of As_2O_3 was added to CNE-2Z cell for succedent experiments. The controls and no drugs of CNE-2Z cells were cultivated for 48 h. Methylation specific PCR was used to detect the change of methylation status of RASSF1A gene. The expression of RASSF1A gene was detected by reverse transcription PCR and Western blot at mRNA and protein level. Results The suppression of cell proliferation by As_2O_3 was time and dose-dependent. After being treated with As_2O_3,the IC_(50) values of As_2O_3 were (1.50±0.05), (1.09±0.13), (0.65±0.04) μmol/L at 24, 48, and 72 h, respectively. As_2O_3 also arrested CNE-2Z cells in G2/M phase of cell cycle. After the effect of As_2O_3, the methylation of RASSF1A gene became weaker by increasing the concentration of As_2O_3; and the expression of RASSF1A gene became stronger at mRNA and protein level. Between different concentration of As_2O_3 group and no drugs group, the differences had statistical significance (P<0.05). Along with increasing the concentration of As_2O_3, the expression of RASSF1A gene became stronger at mRNA and protein level, the methylation of RASSF1A gene became weaker and weaker. Conclusions As_2O_3 can activate the expression of RASSF1A gene to inhibit the cell cyc]e progress of uasopharyngeal carcinoma cell line.
