The mechanism and treatment phases chosen of glycine for inhibition lipopolysaccharide induced Kupffer cells activation.
- Author:
Zuo-jin LIU
1
;
Hai-bo YOU
;
Xu-hong LI
;
Xian-feng CHEN
;
Hai-zhong LIU
;
Yong PENG
;
Chang-an LIU
;
Jian-ping GONG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; Drug Interactions; Glycine; administration & dosage; pharmacology; Interleukin-1 Receptor-Associated Kinases; Intracellular Signaling Peptides and Proteins; genetics; metabolism; Kupffer Cells; drug effects; metabolism; Lipopolysaccharides; pharmacology; Male; Mice; Mice, Inbred BALB C; NF-kappa B; metabolism; Protein-Serine-Threonine Kinases; genetics; metabolism; RNA, Messenger; metabolism; Time Factors; Tumor Necrosis Factor-alpha; metabolism
- From: Chinese Journal of Surgery 2006;44(3):189-192
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the possible mechanism and optimal treatment phase of glycine for inhibition lipopolysaccharide (LPS) induced Kupffer cells (KCs) activation.
METHODSThe KCs were isolated from 40 BALB/c mice and divided into four groups: the endotoxin group, the prevention group, the early treatment group and the later treatment group (n = 10). The endotoxin group was treated with 10 mg/L LPS, and in the other three groups, glycine (1 mmol/L) was given 24 h before, or at 0 h or 4 h respectively after LPS stimulation. At 0 h, 1 h, 2 h, 6 h and 12 h after LPS stimulation, the mRNA levels and protein expression of interleukin-1 receptor associated kinase-4 (IRAK-4) were determined by reverse transcription polymerase chain reaction (RT-PCR) and Western blot respectively, and nuclear factor-kappaB (NF-kappaB) activities as well as tumor necrosis factor alpha (TNF-alpha) levels were also detected by enzyme-linked immunosorbent assay (ELISA).
RESULTSThe climax values of IRAK-4, NF-kappaB and TNF-alpha were significantly higher in the endotoxin group and the later treatment group than that in the other two groups (t = 3.17, 4.33, 2.47, 126.73, P < 0.01).
CONCLUSIONThe results indicated that prophylactic or simultaneous treatment with glycine could effectively inhibit LPS-induced KCs activation by inhibiting IRAK-4 expression.