Saponins isolated from roots of Chlorophytum borivilianum reduce acute and chronic inflammation and histone deacetylase.

Anirudha A Lande; Shirishkumar D Ambavade; E-mail: Shirishkumar77@yahoocom; Uma S Swami; Prafulla P Adkar; Prashant D Ambavade; Arun B Waghamare

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Saponins isolated from roots of Chlorophytum borivilianum reduce acute and chronic inflammation and histone deacetylase.

Author: Anirudha A LANDE ¹ ; Shirishkumar D AMBAVADE ² ; E-mail: SHIRISHKUMAR77@YAHOO.COM. ; Uma S SWAMI ¹ ; Prafulla P ADKAR ¹ ; Prashant D AMBAVADE ³ ; Arun B WAGHAMARE ³
Author Information

1. Department of Pharmacology, JSPM's Jayawantrao Sawant College of Pharmacy and Research, University of Pune, Hadapasar, Pune-411028, India.
2. Department of Pharmacology, JSPM's Jayawantrao Sawant College of Pharmacy and Research, University of Pune, Hadapasar, Pune-411028, India
3. Haffkine Biopharmaceutical Corporation Ltd., Pimpri, Pune-411018, India.
Publication Type:Journal Article
MeSH: Animals; Anti-Inflammatory Agents; pharmacology; Arthritis, Experimental; drug therapy; Female; Histone Deacetylase Inhibitors; pharmacology; Histone Deacetylases; metabolism; Liliaceae; chemistry; Male; Plant Roots; chemistry; Rats; Rats, Wistar; Saponins; pharmacology; therapeutic use; toxicity
From: Journal of Integrative Medicine 2015;13(1):25-33
CountryChina
Language:English
Abstract:
OBJECTIVEThe roots of Chlorophytum borivilanum are used in traditional medicine for the treatment of arthritis and inflammation. The aim of the work was to evaluate the anti-inflammatory activity of isolated saponins from Chlorophytum borivilianum (ISCB).
METHODSThe ISCB was screened using the carrageenan-induced paw edema, histamine-induced paw edema, cotton pellet-induced granuloma, and Freund's adjuvant-induced arthritis in rats at orally administered doses of 3, 10, and 30 mg/kg. Effect of ISCB on histone deacetylase (HDAC) level was measured by the HDAC assay at the highest dose (30 mg/kg).
RESULTSThe results showed that the ISCB significantly reduced carrageenan-induced inflammation, histamine-induced inflammation, cotton pellet-induced granuloma and Freund's adjuvant-induced arthritis in rats. The ISCB at a dose of 30 mg/kg significantly inhibited HDAC level in rat paw tissue.
CONCLUSIONIt is concluded that saponins isolated from roots of C. borivilianum possess anti-inflammatory and anti-arthritic properties. ISCB may act by inhibiting histamine, prostaglandin and HDAC. This suggests that ISCBs have potential for therapeutic use in the treatment of inflammation and arthritis.