Detection of Aichi virus in stool samples from children in Lanzhou
10.3760/cma.j.issn.1003-9279.2010.01.005
- VernacularTitle:在兰州地区儿童粪便中检测 Aichi virus
- Author:
Bo-Wen LI
1
;
Sha LIANG
;
Jin-Xin XIE
;
Jie LI
;
Bei LAN
;
Yong-Qing LI
;
Hui-Ying LI
;
Wei-Xia CHENG
;
Dan-Di LI
;
Yu JIN
;
Zhao-Jun DUAN
Author Information
1. 兰州大学第一临床医学院
- Keywords:
RNA,viral;
Diarrhea,infant;
Epidemiology
- From:
Chinese Journal of Experimental and Clinical Virology
2010;24(1):11-13
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identification and analysis Aichi virus from diarrhea and normal children in Lanzhou, and discuss the relationship between Aichi virus and Infant Diarrhea. Methods According to the literature published data, Using RT-PCR method to amplified Aichi virus 3CD fragment and the positive products were sequenced and determined, and made the alignment analysis between the nucleotide sequences of the amplified fragment with the known sequence of this virus. Results There was 1 case detection of Aichi virus in the 46 hospitalized children with diarrhea and 299 children with diarrhea out-patients specifically, Overall detection rate was 0.06% , and there was no Aichi virus was detected in normal control children. 2 viral 3CD gene and the known reference strains of nucleotide sequences were 97% , while phylogenetic analysis showed that genotype of 2 viral belongs to the B. Conclusions There existed B Genotype of Aichi virus in China, and more research is needed to clarified the etiology and epidemiology of Aichi virus characteristics.
