Studies about the level of CD4+ CD25+ regulatory T cells and relation between expression of Foxp3 and CD127 in peripheral blood of chronic HBV infection.
- Author:
Min DENG
1
;
Ming-Hui LI
;
Shun-Ai LIU
;
Feng LIU
;
Yan SANG
;
Shu-Jing SONG
;
Shu-Feng ZANG
;
Xiao-Ping GUAN
;
Xie YAO
;
Xiao-Ping WU
;
Jun CHENG
;
Dao-Zhen XU
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Female; Forkhead Transcription Factors; blood; genetics; immunology; Hepatitis B virus; immunology; Hepatitis B, Chronic; genetics; immunology; virology; Humans; Interleukin-2 Receptor alpha Subunit; blood; immunology; Interleukin-7 Receptor alpha Subunit; blood; genetics; immunology; Male; Middle Aged; T-Lymphocytes, Regulatory; immunology; Young Adult
- From: Chinese Journal of Experimental and Clinical Virology 2010;24(1):21-23
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the level of CD4+ CD25+ Foxp3+ regulatory T cells and observe relation between expression of Foxp3 and CD127 in peripheral blood of chronic HBV infection.
METHODSCD4+ CD25+ Foxp3+ and CD4+ CD25+ CD127low Treg in peripheral blood from 34 patients of immune tolerance stage, 26 patients of immune clearance stage and 31 patients of non-active status were quantitatively analyzed by flow cytometry.
RESULTSImmune tolerance group presented a higher fraction of CD4+ CD25+ Foxp3+ and CD4+ CD25+ CD127low Treg than non-active group in chronic HBV infection (Z = -2.693, P = 0.007 and t = 3.251, P = 0.002), and HBV positive group also presented a higher fraction than non-active group (t = 2.266, P = 0.026 and t = 3.208, P = 0.002), But ALT normal group is similar to ALT abnormal group (P > 0.05). In this study, the relation between expression of CD127low and Foxp3+ from CD4+ CD25+ regulatory T cells was observed, and CD4+ CD25+ CD127low Treg presented a higher fraction than CD4+ CD25+ Foxp3+ Treg.
CONCLUSIONPeripheral Treg in HBV active replication group is higher than HBV negative group of chronic HBV infection. Expression of CD127low is consistent with Foxp3+ in CD4+ CD25+ regulatory T cells, but the former is significantly higher than the latter.
