Studies about the level of CD4~+CD25~+ regulatory T cells and relation between expression of Foxp3 and CD127 in peripheral blood of chronic HBV infection
10.3760/cma.j.issn.1003-9279.2010.01.008
- VernacularTitle:慢性HBV感染者调节性T细胞水平及Foxp3与CD127表达关系的研究
- Author:
Min DENG
1
;
Ming-Hui LI
;
Shun-Ai LIU
;
Feng LIU
;
Yan SANG
;
Shu-Jing SONG
;
Shu-Feng ZANG
;
Xiao-Ping GUAN
;
Yao XIE
;
Xiao-Ping WU
;
Jun CHENG
;
Dao-Zhen XU
Author Information
1. 江西省南昌大学第一附属医院
- Keywords:
Hepatits B,chronic;
T-lymphocytes;
Receptors,interleukin-7;
Viral load
- From:
Chinese Journal of Experimental and Clinical Virology
2010;24(1):21-23
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the level of CD4~+CD25~+Foxp3~+ regulatory T cells and observe relation between expression of Foxp3 and CD127 in peripheral blood of chronic HBV infection. Methods CD4~+CD25~+Foxp3~+and CD4~+CD25~+CD127~(low) Treg in peripheral blood from 34 patients of immune tolerance stage, 26 patients of immune clearance stage and 31 patients of non-active status were quantitatively analyzed by flow cytometry. Results Immune tolerance group presented a higher fraction of CD4~+CD25~+Foxp3~+ and CD4~+CD25~+CD127~(low) Treg than non-active group in chronic HBV infection(Z= -2.693, P=0.007 and t=3.251, P=0.002 ), and HBV positive group also presented a higher fraction than non-active group(t=-2.266,P=0.026 and t=3.208,P=0.002), But ALT normal group is similar to ALT abnormal group(P>0.05). In this study, the relation between expression of CD127~(low) and Foxp3~+ from CD4~+ CD25~+ regulatory T cells was observed, and CD4~+ CD25~+ CD127~(low) Treg presented a higher fraction than CD4~+CD25~+ Foxp3~+ Treg. Conclusion Peripheral Treg in HBV active replication group is higher than HBV negative group of chronic HBV infection. Expression of CD127~(low) is consistent with Foxp3~+ in CD4~+CD25~+ regulatory T cells, but the former is significant higher than the later.
