The expression of CD95 and activated antigens in peripheral blood lymphocyte subsets in patients with hand foot and mouth disease (HFMD)
10.3760/cma.j.issn.1003-9279.2010.01.012
- VernacularTitle:手足口病患者外周血淋巴细胞亚群激活与CD95表达的检测与意义
- Author:
Ming-Li ZHU
1
;
Song-Ping ZHANG
;
He-Qian CAO
;
Wei-Feng LIANG
;
Jian-Hua YU
;
Guo-Qiang LOU
Author Information
1. 杭州市第六人民医院
- Keywords:
Hand,foot and mouth disease;
T- lymphocytes;
HLA-DR Antigens;
Antigens,CD95
- From:
Chinese Journal of Experimental and Clinical Virology
2010;24(1):33-35
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of CD95 and special marker for activation of peripheral blood lymphocytes in patients with hand foot and mouth disease (HFMD) and its significance. Methods Immunofluorescent two-color flow cytometry was used to study the expression of CD95 and HLA-DR on lymphocytes in 58 patients with HFMD and 34 normal controls. Results Expression of CD3~+ T cells was significantly lower in patients (63.82±7.74)% than that in controls (P<0.001), meanwhile the expression of CD4~+ T cells was (34.29±7.33 ) %, significantly lower than that of the controls (P<0.005). The percentage of lymphocytes expressing HLA-DR in patients was(23.77±5.78)%, significantly higher than that of the controls (P<0.005 ). Significant difference was observed in the expression of HLA- DR on CD8~+ T cells in patients(1.34±1.12)% as compared with controls (P<0.005). No significant difference in the expression of CD95 on lympnocytes was observed between patients and the controls (P>0.05). Conclusion The findings support that cellular immunodeficiency exists in patients and that lymphocytes were abnormally activated in the patients. The activation of peripheral blood T lymphocytes in patients mainly involves CD8~+ subset and it may play an important role in the immune response to antiviral infection.
