Study on the relations between hepatitis B virus large surface protein (LHBs) and HBV YMDD mutation.
- Author:
Li-Jun WANG
1
;
Yan SUN
;
Hai-Ping FU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Amino Acid Motifs; DNA-Directed DNA Polymerase; chemistry; genetics; Female; Hepatitis B; blood; virology; Hepatitis B virus; chemistry; enzymology; genetics; physiology; Humans; Male; Middle Aged; Mutation, Missense; Viral Envelope Proteins; blood; Viral Proteins; chemistry; genetics; Virus Replication; Young Adult
- From: Chinese Journal of Experimental and Clinical Virology 2010;24(1):48-49
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the Relations between hepatitis B virus large surface protein (LHBs) and HBV YMDD mutation, by detecting the hepatitis B virus large protein (LHBs) in Serum of patients with hepatitis B virus YMDD mutation.
METHODSSelected patients after lamivudine treatment, to HBV YMDD mutation detection, then detected LHBs and HBV DNA in Serum of all patients. Serum HBV-DNA was quantitively detected by using real-time polymerase chain reaction (RT-PCR), the LHBS was detected by using enzyme linked immunosorbent assay (ELISA).
RESULTSThere was positive correlation between the A value of LHBs and HBV DNA level in 65 cases of mutation patients (r = 0.961). There was positive correlation between the A value of LHBs and HBV DNA level in 75 cases of non-mutation patients (r = 0.954).
CONCLUSIONThere were positive correlation between the A value of LHBs and HBV DNA level in non-mutation patients and mutation patients, showed that LHBs can partially reflect the level of viral replication in patients, but not the impact of hepatitis B virus YMDD mutation.
