Construction and evaluation of hepatitis C virus (HCV) DNA vaccine containing E_2-gAD fusion gene

VernacularTitle:HCV E_2-gAD融合DNA疫苗的构建及其细胞免疫效果评价
Author: Bo WEN ¹ ; Yao DENG ; Wen-Jie TAN ; Xiao YING ; Ji-Ming GAO ; Li RUAN
Author Information

1. 温州医学院
Keywords: Hepacirirus; Adiponectin; Vaccine,DNA; Viral envelope proteins
From: Chinese Journal of Experimental and Clinical Virology 2010;24(1):53-55
CountryChina
Language:Chinese
Abstract: Objective To rational design HCV DNA vaccine candidates and evaluate their specific immunity to HCV in mice. Methods We design to construct two DNA vaccine candidates, one consists of E_2 (the envelope glycoprotein 2 of HCV) gene only, the second consists of E_2-gAD (Globular Domain of Human Adiponectin) fusion gene via overlapping PCR. Confirm the expression of the DNA vaccines by Western blotting, and then vaccinated by injection of DNA vaccines with gene electrotransfer (GET) in BALB/c mice. The immune response was measured by IFN-gamma ELISPOT. Results The DNA vaccine candidate consists of E_2-gAD could effectively express in vitro , and it could induced a higher anti-HCV T cell response in mice than the one consists of E_2 only. Conclusion The HCV DNA vaccine consists of E_2-gAD fusion can increase the immunity of the E_2 to some extend, and the research paved a way to develop and optimize the novel HCV DNA vaccine.