Construction and identification of a vector inserted with complete genome of poliovirus strain Sabin I.
- Author:
Hong-Hui SHEN
1
;
Jun HOU
;
Yan HU
;
Bing-Ke BAI
;
Zhi-Jie WANG
;
Xiang-Hui YU
;
Wei KONG
;
Ze LIU
;
Pan-Yong MAO
Author Information
- Publication Type:Journal Article
- MeSH: Cloning, Molecular; Genetic Vectors; genetics; Genome, Viral; Mutation; Poliovirus; genetics
- From: Chinese Journal of Experimental and Clinical Virology 2010;24(1):59-61
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a vector inserted with complete genome of poliovirus strain Sabin I.
METHODSThe 3 fragments of the complete genome of Sabin I was amplified and cloned to pEASY-T3 by molecular biological technology. These cloned pEASY-T3 were then digested by Restriction enzymes and ligated to pWSK29 step by step and identified.
RESULTSThe complete genome of poliovirus strain Sabin I was successfully cloned into vector pWSK29 with 9 nucleotide mutations.
CONCLUSIONThe complete genome plasmid was constructed and it provided a basis for further research of the function of Sabin I.
