Construction and identification of a vector inserted with complete genome of poliovirus strain Sabin Ⅰ
10.3760/cma.j.issn.1003-9279.2010.01.021
- VernacularTitle:脊髓灰质炎病毒疫苗株Sabin Ⅰ全基因序列克隆的构建及鉴定
- Author:
Hong-Hui SHEN
1
;
Jun HOU
;
Yan HU
;
Bing-Ke BAI
;
Zhi-Jie WANG
;
Xiang-Hui YU
;
Wei KONG
;
Ze LIU
;
Pan-Yong MAO
Author Information
1. 解放军第302医院
- Keywords:
Poliovirus;
Vaccine;
Cloning,molecular;
Mutation
- From:
Chinese Journal of Experimental and Clinical Virology
2010;24(1):59-61
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a vector inserted with complete genome of poliovirus strain Sabin Ⅰ. Methods The 3 fragments of the complete genome of Sabin Ⅰ was amplified and cloned to pEASY-T3 by molecular biological technology. These cloned pEASY-T3 were then digested by Restriction enzymes and ligated to pWSK29 step by step and identified. Results The complete genome of poliovirus strain Sabin Ⅰ was successfully cloned into vector pWSK29 with 9 nucleotide mutations. Conclusion The complete genome plasmid was constructed and it provided a basis for further research of the function of Sabin Ⅰ.
