Transient expression and identification of gene P and NP of NDV LaSota strain in two different cells
10.3760/cma.j.issn.1003-9279.2010.01.022
- VernacularTitle:NDV LaSota株P、NP蛋白基因表达载体的构建及鉴定
- Author:
Yuan ZHOU
1
;
Run-Qing JIA
;
Zhi-Ping TENG
;
Xiao-Mei ZHANG
;
Yi ZENG
Author Information
1. 北京工业大学
- Keywords:
Newcastle disease virus;
Viral protein;
Reverse genetics manipulation system
- From:
Chinese Journal of Experimental and Clinical Virology
2010;24(1):62-64
- CountryChina
- Language:Chinese
-
Abstract:
Objective To Construction of P and NP genes eukaryotic expression vectors of Newcastle Disease Virus LaSota strain,study its reverse genetics and functional genome of NDV. Methods P, NP genes were amplified and cloned into pGEM-T easy vector and then subcloned into pcDNA3.1 (+) expression vector respectively, the recombinant plasmids were named pcDNA3.1(+)-P and pcDNA3.1 (+)-NP, Recombinant plasmids were transfected into 293 and BHK-21 cells respectively and were detected using IE and Western blot analysis. Results Expression of P, NP genes were detected and confirmed by the IE and WB analysis. Conclusion The recombinant eukaryotic plasmids pcDNA3.1 (+)-P, pcDNA3.1 (+)-NP were expressed in 293 and BHK-21 cells successfully. This research may be helpful for further study of reverse genetics and functional genome of NDV.
