Experimental research on anti-respiratory syncytial virus effect in vitro of earthworm coelomic fluid

VernacularTitle:蚯蚓体腔液体外抗呼吸道合胞病毒的实验研究
Author: Fang LI ¹ ; Jian-Hua WANG ; Jian-Lin ZHANG ; Ru-Gang ZHONG ; Bo NIU
Author Information

1. 山西医科大学
Keywords: Oligachaeta; Antiviral agents; Respiratory syncytial viruses
From: Chinese Journal of Experimental and Clinical Virology 2010;24(2):116-118
CountryChina
Language:Chinese
Abstract: Objective To explore the antivirus function in vitro of earthworm coelomic fluid(ECF) by researching its effect on inhibiting respiratory syncytial virus(RSV).Methods By the method of Hep-2 cell culture and using ribavirin as a positive control,the anti-RSV effect of ECF was investigated by observing cytopathic effect (CPE) with MTT colorimetric assay.Results In Hep-2 cells,the CC50 of ECF and ribavirin were 3.11 mg/ml and 1.35 mg/ml separately.In the experiment of ECF directly killing RSV,the IC50 of ECF was 184.1 μg/ml,SI was 16.87;In the experiment of ECF preventing RSV invasion,no antiviral function of ECF within the experimental concentration range was observed;In the experiment of ECF inhibiting RSV replication,the IC50 of ECF was 1555.8 μg/ml,SI was 1.99.Conclusion ECF couldn't prevent virus from invading into host cell,but showed direct killing-virus function and inhibition of the virus replication.