Preparation of vero cell-adapted influenza H5N1 virus strain by genetic reassortment.
- Author:
Juan SHEN
1
;
Ming-Bo SUN
;
Lei MA
;
Shao-Hui SONG
;
Wei-Dong LI
;
Shu-De JIANG
;
Jing-Si YANG
;
Guo-Yang LIAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Viral; immunology; Cercopithecus aethiops; Chick Embryo; Influenza A Virus, H5N1 Subtype; genetics; immunology; Influenza Vaccines; genetics; immunology; Recombination, Genetic; Vero Cells
- From: Chinese Journal of Experimental and Clinical Virology 2010;24(2):119-121
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo prepare Vero cell-adapted influenza H5N1 virus strain by Genetic Reassortment and produce influenza H5N1 vaccine using Vero cell as a substrate.
METHODSEmbryonated specific pathogen-free (SPF) hen's eggs and Vero cells were co-infected with Vero cell-adapted influenza virus A/Yunnan/1/2005 Va(H3N2) and A/Anhui/1/2005 (H5N1) via reverse genetics. The reassortant was screened with goat antibody against strain A/Yunnan/1/2005 Va(H3N2) and identified for subtype by hemagglutination-inhibition (HI) assays and gene analysis of HA and NA.
RESULTSA Vero cell-adapted influenza H5N1 virus strain was obtained, and there was no significant difference in serum antibody titers of monovalent inactivated vaccine reassorted before and after (F = 0.857, P > 0.05).
CONCLUSIONThe Vero cell-adapted influenza virus of epidemic strain may be reassortment between Vero cell-adapted and epidemic strains.
