Preparation of Vero cell-adapted influenza HSN1 virus strain by genetic reassortment
10.3760/cma.j.issn.1003-9279.2010.02.014
- VernacularTitle:遗传重配获得H5N1流感病毒Vero细胞适应株
- Author:
Juan SHEN
1
;
Ming-Bo SUN
;
Lei MA
;
Shao-Hui SONG
;
Wei-Dong LI
;
Shu-De JIANG
;
Jing-Si YANG
;
Guo-Yang LIAO
Author Information
1. 中国医学科学院北京协和医学院医学生物学研究所
- Keywords:
Influenza A virus;
Reassortmentation genetic;
Vero cells
- From:
Chinese Journal of Experimental and Clinical Virology
2010;24(2):119-121
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare Vero cell-adapted influenza H5N1 virus strain by Genetic Reassortment and produce influenza H5N1 vaccine using Veto cell as a substrate.Methods Embryonated specific pathogen-free (SPF) hen's eggs and Veto cells were co-infected with Vero cell-adapted influenza virus A/Yunnan/1/2005 Va(H3N2) and A/Anhui/1/2005 (H5N1) via reverse genetics.The reassortant was screened with goat antibody against strain A/Yunnan/1/2005 Va(H3N2) and identified for subtype by hemagglutination-inhibition (HI) assays and gene analysis of HA and NA.Results A Vero cell-adapted influenza H5N1 virus strain was obtained,and there was no significant difference in serum antibody titers of monovalent inactivated vaccine reassorted before and after(F=0.857,P>0.05).Conclusion The Vero cell-adapted influenza virus of epidemic strain may be reassortment between Vero cell-adapted and epidemic strains.