Preparation of Vero cell-adapted influenza HSN1 virus strain by genetic reassortment

VernacularTitle:遗传重配获得H5N1流感病毒Vero细胞适应株
Author: Juan SHEN ¹ ; Ming-Bo SUN ; Lei MA ; Shao-Hui SONG ; Wei-Dong LI ; Shu-De JIANG ; Jing-Si YANG ; Guo-Yang LIAO
Author Information

1. 中国医学科学院北京协和医学院医学生物学研究所
Keywords: Influenza A virus; Reassortmentation genetic; Vero cells
From: Chinese Journal of Experimental and Clinical Virology 2010;24(2):119-121
CountryChina
Language:Chinese
Abstract: Objective To prepare Vero cell-adapted influenza H5N1 virus strain by Genetic Reassortment and produce influenza H5N1 vaccine using Veto cell as a substrate.Methods Embryonated specific pathogen-free (SPF) hen's eggs and Veto cells were co-infected with Vero cell-adapted influenza virus A/Yunnan/1/2005 Va(H3N2) and A/Anhui/1/2005 (H5N1) via reverse genetics.The reassortant was screened with goat antibody against strain A/Yunnan/1/2005 Va(H3N2) and identified for subtype by hemagglutination-inhibition (HI) assays and gene analysis of HA and NA.Results A Vero cell-adapted influenza H5N1 virus strain was obtained,and there was no significant difference in serum antibody titers of monovalent inactivated vaccine reassorted before and after(F=0.857,P＞0.05).Conclusion The Vero cell-adapted influenza virus of epidemic strain may be reassortment between Vero cell-adapted and epidemic strains.