Effects of local hyperthermia on maturation of Langerhans cells in HPV-infected skin
10.3760/cma.j.issn.1003-9279.2010.02.015
- VernacularTitle:温热对HPV感染皮肤LC成熟功能的影响
- Author:
Xiao-Dong LI
1
;
Xing-Hua GAO
;
Yu-Xiao HONG
;
Yu-Bo LIU
;
Li-Li ZHU
;
Ya-Kun WANG
;
Xiao-Qin WANG
;
Wei HUO
;
Li ZHANG
;
Hong-Duo CHEN
Author Information
1. 沈阳医学院奉天医院
- Keywords:
Hyperthermia,induced;
Papillomavirus,human;
Langerhans cells;
Dendritic cells;
Condyloma accuminata
- From:
Chinese Journal of Experimental and Clinical Virology
2010;24(2):122-124
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the possible mechanism of local hyperthermia in the treatment of warts through detecting the differences in CD1a/CD83 of Langerhans cells(LCs) in émigrés from HPV-infected skin,as compared to normal skin.Methods Confocal microscopy were performed on Condyloma Aecuminatum (CA) and normal skin;Freshly taken biopsies of CA and normal skin were subjected to surface heating at 37℃,42T℃ and 45℃ respectively,for 30mins.Flow cytometry was used to determine the CD1a/CD83 changes of LCs in émigrés from CA and normal skin.Results By confocal microscopic observation,there were practically no CD1a~+ LCs that expressed CD83 in the epidermis of both normal skin and CA.The proportions of CD1a~+/CD83~+ LCs were significantly increased with increased temperatures in émigrés from both normal skin and CA.At each given temperature,the numbers of LCs in émigrés from CA were greater than those from normal skin.Conclusion Local hyperthermia can promote migration and maturation of LCs in HPV-infected skin and accordingly stimulate the immune system to treat warts.
