Research of PD-1 expression in CD8~+ T cell of Peripheral blood with HBV-associated acute-onchronic liver failure
10.3760/cma.j.issn.1003-9279.2010.02.016
- VernacularTitle:PD-1受体在HBV-ACLF患者外周血CD8~+T细胞上的表达研究
- Author:
Xiao-Yan LIU
1
;
Feng SHI
;
Hong ZHAO
;
Hui-Fen WANG
Author Information
1. 解放军第302医院
- Keywords:
Liver failure;
PD-1;
CD8-Positive T-lymphocytes
- From:
Chinese Journal of Experimental and Clinical Virology
2010;24(2):125-127
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze PD-1 expression in CD8~+T cell of Peripheral blood with HBV-associated acute-on-chronic liver failure and effect on CD8~+T cell.Methods We selected 60 patients with HBV-ACLF and collected their peripheral blood.We analyzed the expressions of PD-1,CD95,perforin,granzyme A,granzyme B,CD107a on CD8~+T lymphocytes and the expression of PD-L1 on monocytes in peripheral blood by using flow cytometry.15 liver cirrhosis patients(LC) and 15 healthy individuals(HC) are control groups.Result PD-1 expression was (1)The PD-1 expression in HBV-ACLF patients was significantly elevated compared with those in HC and lower in improved group than that in invalid group and death group (P＜0.05) and increased from prophase,metaphase to advanced stage (P＜0.05).Moreover.(2)PD-L1 expression on monocytes was positively correlated with disease progression.(P＜0.05).(3)Both PD-1 and CD95 expressions were higher in dead group than those in improved and non-improved groups.Perforin,granzymes and CD107a expressions on CD8~+ T cells significantly increased in dead group compared with those in improved and non-improved groups (P＜0.05).However,PD-1 expressions on these cells were lower,compared with normal persons.Conclusions The expression of PD-1 and PD-L1 in HBV-ACLF patients was positively correlated with disease progression.The elevated PD-1 expression promoted apoptosis of CD8~+T ceils.For HBV-ACLF patients,the PD-1 expression on effector CD8~+T cells was lower than those in other CD8~+T cells,which maybe accounted for the failure to controlling immune injury in liver.
