Research on the methods for titrating respiratory syncytial virus
10.3760/cma.j.issn.1003-9279.2010.02.025
- VernacularTitle:呼吸道合胞病毒感染滴度测定方法的建立与比较
- Author:
Xiao-Bo WANG
1
;
Jin-Sheng HE
;
Yuan-Hui FU
;
Xian-Xian ZHENG
;
Xuan FANG
Author Information
1. 安徽医科大学
- Keywords:
Respiratory syncytial virus,human;
Titrimetry;
Polymerase chain reaction;
Immunoenzyme tests;
Inhibitory concentration 50
- From:
Chinese Journal of Experimental and Clinical Virology
2010;24(2):147-149
- CountryChina
- Language:Chinese
-
Abstract:
Objective Respiratory syncytial virus (RSV) is the most common cause of lower respiratory infection in infants.It is very important to quantitative assay of RSV titer in the study on RSV pathogenesis,candidate vaccine and antiviral treatment.Therefore,we develop Real-time Quantitative PCR (Q-PCR) assay and enzyme immunospots (EIS) for titrating RSV and compare them with traditional 50% tissue culture infectious doses (TCID_(50)).Methods Q-PCR,based upon the RSV-L genes,and EIS were utilized to titrate samples from RSV culture supernatants and RSV infected mouse lungs.Then,the results were compared with TCID_(50).Results For the samples from RSV culture supernatants,the ratio of Q-PCR and EIS (plaque forming unit,pfu) was 10:1 and the ratio of EIS and TCID_(50) was 10:1 when TCID_(50) was converted as pfu.For the samples from RSV infected mouse lungs,the ratio of Q-PCR and EIS was 1000:1 and the ratio of EIS and TCID_(50) was 5:1.Conclusion We have successfully established Q-PCR and EIS to titrate RSV and compared them with TCID_(50).We concluded EIS is a cost-effective method to titrate RSV.
