The expression and preliminary evaluation of HPV6bL2△N360E7E6 fusion protein in E.coli for genital warts
10.3760/cma.j.issn.1003-9279.2010.03.004
- VernacularTitle:用于治疗尖锐湿疣的HPV6型L2△N360E7E6融合蛋白的原核表达及其免疫效果的初步评价
- Author:
Zheng PANG
1
;
Li ZHAO
;
Jiao REN
;
Ring FENG
;
Zhong-Xian ZHANG
;
Wen-Jie TAN
;
Li RUAN
;
Hou-Wen TIAN
Author Information
1. 中国疾病预防控制中心病毒病预防控制所
- Keywords:
Candylomata acuminata;
Papillomavirus,human;
Expressed sequence tags;
Immunity,cellular;
Antibody formation
- From:
Chinese Journal of Experimental and Clinical Virology
2010;24(3):171-174
- CountryChina
- Language:Chinese
-
Abstract:
Objective To express HPV6bL2△N360E7E6 fusion protein in E.coli and preliminarily evaluate its immune effect.Methods Three HPV6b gene fragments,which were L2(1-360 bp),E7 and E6,were fused by overlapping PCR,then were inserted into a prokaryotic expression vector and expressed in E.coli.C57BL/6 mice were immunized with purified fusion protein plus Al(OH)3 and/or CpG adjuvants through intramuscular route,the cellular and humoral immune responses were detected by IFN-γ ELISPOT and ELISA respectively.Results Protein plus CpG adjuvant could induce the strongest cellular immune response to E7 and E6,high antibody titer against L2 could be detected in all immunized groups but there were no significant difference among these groups.Conclutions HPV6bL2△N360E7E6 gene was successfully cloned into pQE30 vector and expressed in E.coli,the fusion protein was also purified and proved that could induce strong cellular and humoral immune responses with appropriate adjuvant in C57 BL/ 6 mice and could be used for future research.
