Construction and experimental study on off-line hybrid bioartificial liver supporting system with human liver cell line
10.3760/cma.j.issn.1003-9279.2010.03.011
- VernacularTitle:人肝细胞离线混合生物人工肝支持系统的构建与实验研究
- Author:
Yi-Hui RONG
1
;
Hong-Ling LIU
;
Shao-Li YOU
;
Wan-Shu LIU
;
Yan HU
;
Jun HOU
;
Pan-Yong MAO
;
Shao-Jie XIN
Author Information
1. 解放军第302医院
- Keywords:
Liver,artificial;
Hepatocytes;
Liver failure
- From:
Chinese Journal of Experimental and Clinical Virology
2010;24(3):193-195
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct an off-line hybrid bioartificial liver supporting system with human liver cell line,and study it's effect on the plasma from patients with liver failure.Methods We established the bioreactor using Psu-2s(Fresenius)cultured with Hep G2 cell transfected with human augmenter of liver regeneration (hALR) gene,then constructed a hybrid bioartificial liver supporting system,at last using the bioartificial liver support system to purify the plasma treated 2 hours with serum bilirubin absorbent,separated from acute on chronic liver failure patients infected by hepatitis B virus.Results Bioreactor was successful constructed.The cell viability in perigastrum of bioreactor is 85.2% and cell propagated rapidly.Before and after treating with bilirubin absorbent,serum total bilirubin was(176.19 ±54.14)μmol/L and(50.1±1 6.85)μmol/L respectively(P=0.0002).While there were no signifcance difference in the level of albumin.urea and glucose.At the begin and end of treatment with bioartificial liver,serum total bilirubin was(50.10±16.85)μmol/L and(30.27±15.02)μmol/L respectively(P=0.000),the urea and albumin increased,urea has significantly difference,but the change of albumin hasn't.Conclusion The off-line hybrid bioartificial liver supporting system with human liver cell line were builded successfully and have synthesis and metabolism functions for acute on chronic liver failure patients.
