Study on the inhibition effect of siRNA on herpes simplex virus type 2 ICP4 gene
10.3760/cma.j.issn.1003-9279.2010.03.013
- VernacularTitle:siRNA对单纯疱疹病毒2型ICP4基因抑制作用的研究
- Author:
Ji-Feng LIU
1
;
Cui-Ping GUAN
;
Xu TANG
;
Ai-E XU
Author Information
1. 浙江省杭州市第三人民医院
- Keywords:
RNA viral;
Herpesvirus 2,human;
Genes,INP4
- From:
Chinese Journal of Experimental and Clinical Virology
2010;24(3):199-201
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the inhibition effect of RNA interference on the IcP4 expression and DNA replication of herpes simplex virus type 2(HSV2).Methods Four pairs of siRNA targeted to HSV2 ICP4 gene and negative control siRNA were synthetized by chemieal method.named as siRNA-1,siRNA-2,siRNA-3,siRNA-4 and siRNA-N respeeticely.HSV2 HG52 was used to attack Vero cell after transfection overnight.Vero cell and supernatant were collected at 1d,2d,3d,4d and 5d after virus attacking.Flurogenic quantitative reverse transcription polymerase chain reaction(FQ-RT-PCR)was used to detect the expression of HSV2 ICP4 mRNA,flurogenic quantitative polymerase chain reaction (FG-PCR) was used to detect the expression of HSV2 DNA and Western-Blot was used to detect the expression of HSV2 ICP4 protein.Results All the four pairs of siRNA could significantly inhibit the expression of HSV2 ICP4 mRNA and protein,especially siRNA-2.The above siRNAs could significantly decrease HSV2 DNA copy number,too.Conclusion siRNAs targeted to HSV2 ICP4 gene could significantly inhibit expression of HSV2 ICP4 mRNA and protein,and decrease HSV2 DNA copy number,suggesting that siRNA can inhibit HSV2 DNA replication through silencing ICP4 gene.
