Occult HBV infection in patients with anti-HBc positive alone
10.3760/cma.j.issn.1003-9279.2010.03.020
- VernacularTitle:抗-HBc单项阳性患者中的隐匿性HBV感染
- Author:
Xiang-Yan HUANG
1
;
Xiao-Di LI
;
Xiang-Juan HUANG
;
Qian SHEN
Author Information
1. 济南军区总医院
- Keywords:
Hepatitis B virus;
Hepatitis antibodies;
Infection control
- From:
Chinese Journal of Experimental and Clinical Virology
2010;24(3):221-223
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study was designed to explore the incidence rate of occuIt HBV infection in patients with anti-HBc positive alone and analyze the possible reasons of occuIt infection.Methods Sera of 183 patients carrying anti-HBc alone(A≤0.1) were collected and real-time PCR was used to select samples with HBV DNA positive.HBV pre-S/S amplification products were obtained by PCR,and clonal sequencing were then used for these samples with HBV DNA positive.Results DNA quantitative results of three samples were greater than 103 copies/ml in 183 samples,with a fraction of 1.6%.Pre-S/S sequencing results of two samples from these three samples were obtained.Point mutations within "a" determinant with Q129R/P mutations and co-existence of the mutant type and wild type were found in the two samples.Conclusions Occult HBV infection existed in samples with anti-HBc alone.Factors contributing to the loss of HBsAg detection by immunoassays include S gene mutations and low levels of cireulating antigen which are below the assay limit of detection.Occult HBV infection not only can lcad to a false clinical diagnosis,but also can result in hematological pollution due to such occult infection of blood donors.
