Development of a rapid test kit for antibody to HIV by nano immunomagnetic lateral flow method

VernacularTitle:HIV抗体纳米免疫磁珠快速检测试剂的研制
Author: Fa-Qing YANG ¹ ; Lee TONY ; Chao-Nan WANG ; Shu-Ye SUN ; Shan-Shan LI ; Hui TIAN
Author Information

1. 天津中新科炬生物制药有限公司
Keywords: HIV antibodies; Nano immunomagnetic particle; Lateral flow; Rapid test
From: Chinese Journal of Experimental and Clinical Virology 2010;24(3):235-237
CountryChina
Language:Chinese
Abstract: Objective To develop a rapid test kit for antibody to HIV by nano immunomagnetic lateral flow method.Methods A rapid test kit was developed by conjugation of the HIV antigen gp41 and gp36 to 200nm super paramagnetic particles by carbodiimide (EDC) and coating of the HIV antigen sp41 and gp36 to nitrocellulose membrane.Then the kit was evaluated with serials of experiments.Results The kit was qualified with examination of national reference panel of anti-HIV antibody for colloidal gold diagnostic kit.The sensitivity was 100% by tested with 20 HIV antibody positive sera,the specificity was 98.5% by tested with 600 HIV antibody negative sera,respectively.The stabilitv of the kit was over 12 month by storage at room temperature.Conclusion A diagnostic kit for antibody to HIV was developed with the advantages of convenience,rapid test,good stability and point of care.