Variation of the V3 loop tip motifs and primary drug resistance of HIV-1 strains

VernacularTitle:HIV-1毒株V3环顶端四肽多态性及原发耐药性研究
Author: Peng DU ¹ ; Guo-Min CHEN ; Ze-Lin LI ; Yi ZENG
Author Information

1. 北京市石景山区疾病预防控制中心
Keywords: HIV; V3 Loop Tip Motifs; Drug resistance,viral
From: Chinese Journal of Experimental and Clinical Virology 2010;24(5):321-323
CountryChina
Language:Chinese
Abstract: Objective To study the characteristics of the variation of the V3 loop tip motifs and the drug resistance in the primary treatment patients. Methods The partial region of the HIV-1 env and pol gene in 51 samples was amplified by nested polymerase chain reaction (PCR) ,purified products were cloned into the vectors, the obtained were analyzed by MEGA soft wares. Results The V3 loop tip motifs had four types in our study ( GPGR, GPGQ, GPGK, GQGR); the study on the drug resistance in primary treatment patients, showed that there were not major resistance associated with PI, and the resistance were minor mutations in protease gene. In the RT region, there were nine resistance mutants were single NRTIs or NNRTIs. Conclusion The GPGR which was the typical western V3 loop tip motifs attained to 44.44%.This results showed that the percentage of primary drug resistance was still low in our study region,suggesting no need for genotyping detection in blood donor patients before primary therapy.