Association of serum HBV DNA level with cytotoxic T lymphocytes in patients with HBV-induced hepatic cirrhosis
10.3760/cma.j.issn.1003-9279.2010.05.003
- VernacularTitle:乙型肝炎肝硬化患者血清HBV DNA水平与细胞毒性T淋巴细胞的关系和意义
- Author:
Xi-Bing GU
1
;
Xiao-Juan YANG
;
Dong WANG
;
Zhong HUA
;
Yue-Qin XU
;
Zhong-Hua LU
Author Information
1. 无锡市传染病医院
- Keywords:
Hepatitis B;
Liver cirrhosis;
DNA;
T-lymphocytes,cytotoxic
- From:
Chinese Journal of Experimental and Clinical Virology
2010;24(5):327-330
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the association of serum HBV DNA level with HBV-specific and nonspecific cytotoxic T lymphocytes (CTL) in patients with HBV-induced hepatic cirrhosis. Methods 120 patients with HBV-induced hepatic cirrhosis who were positive for HBV DNA, HBeAg and human leucocyte antigen (HLA)-A2 were enrolled in this study. The level of HBV DNA was determined by real time fluorescence quantitative polymerase chain reaction (PCR). HBV-specific and nonspecific CTL were detected by flow cytometry. Liver function tests were done in the 120 patients. The 120 patients were divided into group A and B based on their HBV DNA levels. In group A, there were 68 patients with HBV DNA level of 3-4 log10 copy/ml, and in group B, 52 with 5-6 log10 copy/ml. HBV-specific and nonspecific CTL and liver function were compared between the two groups. Results HBV DNA levels were (3. 68 ± 0. 19)and (5.97 ±0. 32) log10 copy/ml in Group A and B respectively with P ＜ 0. 001. HBV-specific CTL was higher in group A (0.33% ±0.04%) than in group B (0.11% ±0.01%) with P ＜0.001. HBV-nonspecific CTL were (11.99% ±1.51% ) and (11.91% ± 1.61% ) in group A and B respectively with P＞ 0. 05. Conclusion The level of serum HBV DNA is related to the levels of HBV-specific CTL in patients with HBV-induced hepatic cirrhosis. Patients with higher HBV DNA had lower levels of HBV-specific CTL,and the damage to liver function was severe because of higher levels of HBV DNA. Patients with lower HBV DNA had higher levels of HBV-specific CTL which predict good anti-viral effect.
