Genetic characterization of measles virus caused the measles outbreak in Xinjiang

VernacularTitle:引起乌鲁木齐成人麻疹暴发的麻疹病毒基因特征分析
Author: Hui-Ling WANG ; Song-Tao XU ; Nai-Ying MAO ; Wen-Bo XU ; Yan ZHANG
Keywords: Measles virus; Sequence analysis; Genotype
From: Chinese Journal of Experimental and Clinical Virology 2010;24(5):337-339
CountryChina
Language:Chinese
Abstract: Objective To analyze the genetic characterizations of wild type measles viruses caused the measles outbreak in Xinjiang. Methods Vero/Slam cell were used for measles viruses isolation from the specimens collected from measles outbreaks patients. Fragment of 676 nucleotide acids of the carboxylend of nucleoprotein gene were amplified using reverse transcription-polymerase chain reation (RT-PCR) method and then the PCR products were directly sequenced and analyzed. Phylogenetic tree was constructed based on 450 nucleotide acids of the N-terminus of nucleoprotein gene, and homological analysis was performed at nucleotide acid level. Results 11 measles viruses were sequenced and all belonged to H1a subgenotype.The nucleotide difference was 0-0. 2% between 11 Xinjiang isolates. And the nucleotide difference was 2.2%-2.4% between Xinjiang isolates and H1 genotype reference strain. Conclusion The Measles viruses causing the measles outbreak in Xinjiang were H1a subgenotype.