Transmission electron microscopy investigation of the denudation of the envelope of influenza virus treated with Nonidet P-40 and the binding influence of antibody
10.3760/cma.j.issn.1003-9279.2010.05.007
- VernacularTitle:Nonidet P-40裂解流感病毒及其对抗体结合影响的透射电镜观察
- Author:
Kong-Xin HU
1
;
Li-Ping ZHANG
;
Rui-Jin PING
;
Xue-Zheng MA
;
Peng-Fei YANG
;
Xiao-Hong SUN
;
Le ZHANG
Author Information
1. 中国检验检疫科学研究院
- Keywords:
Microscopy,electron;
Surface-active agents;
Orthornyxoviridae
- From:
Chinese Journal of Experimental and Clinical Virology
2010;24(5):340-342
- CountryChina
- Language:Chinese
-
Abstract:
Objective Through observing the morphological changes of the prepared influenza viruses (H1N1) treated with the different concentration of Nonidet P-40 solutions and added with antibody against the influenza virus using transmission electron microscopy (TEM), to explore the principle of application of the internal antibody for immunoassay of influenza virus. Methods Through treating the virus samples with serial diluted Nonidet P-40 solutions from 0.01% to 0. 2% and/or not adding antibody against the influenza virus, then investigating the samples by TEM to obtain the morphological changes of the virions. Results The serial images show that the denudation degree of the virions is proportional with the rise of NP-40 concentration, and partly denuded virion image appeared at 0. 1% NP-40 treatment, also under this condition no influence was observed on antibody binding to the virus. Conclusion This work demonstrated the interaction between influenza virion and its antibody under nonionic surfactants existing,which supports the advantages of sample preparation for immunoassay enveloped virus using internal antibody theoretically.
