Clinical significance of measuring hepatitis B virus large surface protein in serum.
- Author:
Nian-yue WANG
1
;
Dai ZHANG
;
Yan-wen GE
;
Wei ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Case-Control Studies; DNA, Viral; blood; genetics; Gene Expression; Hepatitis B; blood; diagnosis; virology; Hepatitis B virus; genetics; isolation & purification; physiology; Humans; Viral Envelope Proteins; blood; genetics; Virus Replication
- From: Chinese Journal of Experimental and Clinical Virology 2010;24(5):349-351
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the significance of serum hepatitis B virus large protein( HBV-LP), HBV-DNA and markers of hepatitis B virus (HBV-M)in the diagnosis of viral replication.
METHODSSerum HBV-DNA level was quantitatively detected using PCR Real-time polymerase chain reaction, HBV-LP was detected by enzyme-linked immunosorbent assay (ELISA) and HBV markers expression were measured by chemiluminescence immunoassay method in 1886 cases of seurm.
RESULTSThe results of hepatitis virus large protein (HBV-LP) detection and the detection results of HBV-DNA was no significant difference (chi2 = 1.142, P > 0.05). HBV-DNA logarithm of copies and A vaule of HBV-LP was a positive correlation (r = 0.487, P < 0.01). HBV-DNA copies of different groups was significantly different from HBV-LP A values (F = 7.772, P < 0.01). The results of HBV-LP and HBV-DNA detected in different patterns of HBV-M were not significantly different. In 36 healthy people,the detecting results of HBV-DNA and HBV-LP are negative.
CONCLUSIONThere is a good correlation between the copies of HBV-DNA and the levels of HBV-LP. HBV-LP expression can reflect the replication of HBV.
