Effect of Itk down regulation on cytokines production in Jurkat cell.
- Author:
Hai-lan YAO
1
;
Feng HE
;
Zong-hui XIAO
;
Ji-sheng HAN
;
Yang-de ZHANG
;
Bo-yun HUANG
;
Zhe-wei LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Proliferation; Cytokines; genetics; immunology; Down-Regulation; Gene Knockdown Techniques; Humans; Interleukin-10; genetics; immunology; Interleukin-2; genetics; immunology; Interleukin-5; genetics; immunology; Jurkat Cells; cytology; enzymology; immunology; Mice; Protein-Tyrosine Kinases; genetics; immunology
- From: Chinese Journal of Experimental and Clinical Virology 2010;24(5):358-361
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of Itk down regulation on Jurkat cell proliferation and inflammatory cytokines production, and provide useful data for Itk as an attractive target for potential drugs.
METHODSThree shRNAs against different region of Itk were constructed and cotransfected with pEGFP-C1-hItk. The shRNA, which can knock down Itk, was selected and packed into lentivirus. After Jurkat cells were transfected with shRNA lentivirus, the change of Itk protein expression, cell proliferation and cytokines production was observed.
RESULTSItk mRNA was reduced about 55% in Jurkat cells transfected with Itk-shRNA1, compared with that in control cells shRNAnon (P < 0.05). Knocking down Itk expression had a profound inhibitory effect on Jurkat cell proliferation. In addition, there was a substantial decrease in level of cytokines, such as IL-2, IL-5, IL-10 and IFN-gamma, produced by cell transfected with Itk-shRNA1.
CONCLUSIONKnocking down Itk expression can inhibit Jurkat cell proliferation and inflammatory cytokines production.
