Development of serological detection assays for human coronavirus HKU1 infection and its application
10.3760/cma.j.issn.1003-9279.2010.05.019
- VernacularTitle:人冠状病毒HKU1血清学检测方法的建立及其初步应用
- Author:
Wei-Min ZHOU
1
;
Wen-Ling WANG
;
Wen-Jie TAN
;
Ling-Lin ZHANG
;
Xiao YIN
;
Rou-Jian LU
;
Hui-Juan WANG
Author Information
1. 中国疾病预防控制中心
- Keywords:
Coronavirus;
Viral proteins;
Antibodies,viral;
Serology
- From:
Chinese Journal of Experimental and Clinical Virology
2010;24(5):376-379
- CountryChina
- Language:Chinese
-
Abstract:
Objective To express the nuclear capsid protein (N protein) and the spike protein (S protein) of HCoV-HKU1, and to develop the corresponding serum assay for antibody detection. Methods The N protein of HCoV-HKU1 was expressed in E. Coli, anti-N antibody assay was established using Western Blotting with turn-based membrane. HCoV-HKU1 S protein was constructed in the eukaryotic expression plasmids, and confirmed by Western Blotting, S antibody assay was established using indirect immunofluorescence assay (IFA). We analyzed anti-S and anti-N antibody among 100 normal adult serum.Results Expression of S and N protein were confirmed;100 normal adult serum were analyzed using the established serological detection assay, in which HCoV-HKU1 S antibody positive rate was 47% , N antibody positive rate was 48% , Both S and N antibodies positive were 21% , Both S and N antibodies negative were 22%. Co-detection S and N antibody was achieved 74% positive rate. Conclusion The methods we established here could be used for serological analysis of HCoV-HKU1. Either detection of HCoV-HKU1 S or N antibodies achieved good results. Higher positive detection rate of anti-S or anti-N antibody was found in the normal adults.
