Preparation and identification of the monoclonal antibodies against programmed death-1 ligant 1.
- Author:
Li-ping SHEN
1
;
Si-yong CHEN
;
Yu GUO
;
Shuang ZHANG
;
Tao BIAN
;
Sheng-li BI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Monoclonal; immunology; Antibody Specificity; Antigens, CD; immunology; B7-H1 Antigen; Enzyme-Linked Immunosorbent Assay; Female; Humans; Hybridomas; Mice; Mice, Inbred BALB C
- From: Chinese Journal of Experimental and Clinical Virology 2010;24(5):380-382
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo prepare monoclonal antibodies (McAbs) against human programmed death-1 ligant 1(PD-L1) and identify its bioactivity.
METHODSWe immunized the BALB/c mice with Thioredoxin-(PD-L1) recombination protein which expressed by prokaryotic system. Prepare hybridoma cell by hybridoma technology and used enzyme-linked immunosorbent assay(ELISA) and Western-blotting assays to select positive hybridoma identify cell. Competition inhibition ELISA was carried out to identify the special bioactivity of antibody.
RESULTS4 hybridoma cell strains which could secrete anti-(PD-L1) antibodies stably were selected. The McAbs has good affinity with its receptor. Purify anti-(PD-L1) with title 1:32 000 was obtained after large quantity preparation. At the same time we obtained 1 cell stain which could secret special anti-Trx McAbs.
CONCLUSIONSWe obtained anti-(PD-L1) McAbs with good bioactivity successfully, which lay the foundation for further study.
