Identification specific T lymphocyte epitopes on E6 protein of human papillomavirus type 18 in mice.
- Author:
Li ZHAO
1
;
Jiao REN
;
Jing FENG
;
Zheng PANG
;
Zhong-Xian ZHANG
;
Wen-Jie TAN
;
Hou-Wen TIAN
;
Li RUAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; CD8-Positive T-Lymphocytes; immunology; DNA-Binding Proteins; immunology; Epitopes, T-Lymphocyte; immunology; Human papillomavirus 18; immunology; Humans; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Oncogene Proteins, Viral; immunology; Papillomavirus Vaccines; immunology
- From: Chinese Journal of Experimental and Clinical Virology 2010;24(6):406-408
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify specific T lymphocyte epitope on E6 protein of human papillomavirus type 18 in mice.
METHODSInfection with one recombinant vaccinia virus rVVJ18 E7, E6 respectively in C57 BL/6 and BALB/c mice, specific cellular immune responses were detected by ELISPOT or intracellular cytokine stainings by using a series of overlapping synthetic peptides covering full length of the amino acid sequence of E6 and E7 proteins or various truncated peptides.
RESULTSThe rVVJ18 E7, E6 generated significant E6 specific T-cell immune responses in vaccinated mice. Mapping of the epitope of E6 revealed that the peptides E6(67-75 ( KCIDFYSRI) and E6(60-68) (IPHAAGHKC) presented respectively by C57BL/6 and BALB/c mice were the optimal peptides to activate E6-specific CD8+ T lymphocytes. However no positive cellular immune responses stimulated with various E7 peptides were detected by ELISPOT in immunized mice.
CONCLUSIONSTwo specific T lymphocyte epitopes were identified on E6 protein in C57BL/6 and BALB/c mice, which will provide the basis to evaluate cellular immune response elicited by HPV18 E6 protein based vaccine.
