Identification specific T lymphocyte epitopes on E6 protein of human papillomavirus type 18 in mice
10.3760/cma.j.issn.1003-9279.2010.06.004
- VernacularTitle:人乳头瘤病毒18型E6、E7蛋白T细胞表位筛选
- Author:
Li ZHAO
1
;
Jiao REN
;
Jing FENG
;
Zheng PANG
;
Zhong-Xian ZHANG
;
Wen-Jie TAN
;
Hou-Wen TIAN
;
Li RUAN
Author Information
1. 中国疾病预防控制中心病毒病预防控制所
- Keywords:
Papillomavirus,human;
Epitopes;
Vaccinia virus
- From:
Chinese Journal of Experimental and Clinical Virology
2010;24(6):406-408
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify specific T lymphocyte epitope on E6 protein of human papillomavirus type 18 in mice. Methods Infection with one recombinant vaccinia virus rVVJ18 E7, E6 respectively in C57 BL/6 and BALB/c mice, specific cellular immune responses were detected by ELISPOT or intracellular cytokine stainings by using a series of overlapping synthetic peptides covering full length of the amino acid sequence of E6 and E7 proteins or various truncated peptides. Results The rVVJ18 E7, E6 generated significant E6 specific T-cell immune responses in vaccinated mice. Mapping of the epitope of E6 revealed that the peptides E667-75 (KCIDFYSRI) and E660-68 ( IPHAAGHKC ) presented respectively by C57BL/6 and BALB/c mice were the optimal peptides to activate E6-specific CD8 + T lymphocytes. However no positive cellular immune responses stimulated with various E7 peptides were detected by ELISPOT in immunized mice. Conclusions Two specific T lymphocyte epitopes were identified on E6 protein in C57BL/6 and BALB/c mice, which will provide the basis to evaluate cellular immune response elicited by HPV18 E6 protein based vaccine.
