Study on antineoplastic effect of earthworm coelomic fluid in vitro.
- Author:
Jian-Hua WANG
1
;
Tao GUAN
;
Xiao-Yuan WANG
;
Zhi-Zhen LIU
;
Jian-Lin ZHANG
;
Xiu-Wei WANG
;
Guo-Liang WANG
;
Ru-Gang ZHONG
;
Bo NIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antineoplastic Agents; pharmacology; Apoptosis; drug effects; Body Fluids; chemistry; COS Cells; Cell Growth Processes; drug effects; Cell Line; Cell Line, Tumor; Cercopithecus aethiops; Oligochaeta; chemistry; PC12 Cells; Rats
- From: Chinese Journal of Experimental and Clinical Virology 2010;24(6):409-411
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the antineoplastic effect in vitro of earthworm coelomic fluid (ECF)on growth inhibition and its mechanism for the tumor cell lines Siha, SW480, Colo205 and PC12.
METHODSMTT colorimetric assay, flow cytometry and morphological analysis were used to test its antitumor activity on tumor cell lines and normal cell line Cos7 in vitro.
RESULTSECF can inhibit the cell growth of Siha, SW480, Colo205, PC12 and Cos7. But different tumor cell lines showed different sensitivity.
CONCLUSIONEFC can significantly inhibit the proliferation of tumor cells in vitro by inducing tumor cells apoptosis.
