Study on antineoplastic effect of earthworm coelomic fluid in vitro
10.3760/cma.j.issn.1003-9279.2010.06.005
- VernacularTitle:蚯蚓体腔液体外抗肿瘤作用的实验研究
- Author:
Jian-Hua WANG
1
;
Tao GUAN
;
Xiao-Yuan WANG
;
Zhi-Zhen LIU
;
Jian-Lin ZHANG
;
Xiu-Wei WANG
;
Guo-Liang WANG
;
Ru-Gang ZHONG
;
Bo NIU
Author Information
1. 北京工业大学
- Keywords:
Oligochaeta;
Antineoplasticagents;
Cell line
- From:
Chinese Journal of Experimental and Clinical Virology
2010;24(6):409-411
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the antineoplastic effect in vitro of earthworm coelomic fluid (ECF) on growth inhibition and its mechanism for the tumor cell lines Siha, SW480, Colo205 and PC12.Methods MTT colorimetric assay, flow cytometry and morphological ananlysis were used to test its antitumor activity on tumor cell lines and normal cell line Cos7 in vitro. Results ECF can inhibit the cell growth of Siha, SW480, Colo205, PC12 and Cos7. But different tumor cell lines showed different sensitivity. Conclusion EFC can significantly inhibit the proliferation of tumor cells in vitro by inducing tumor cells apoptosis.
