Immunogenicity of plasmid DNA and adenoviral vectors encoding HIV-1 subtype B env gene.
- Author:
Hai-Ru YANG
1
;
Ling-Fei ZHANG
;
Xia FENG
;
Shuang-Qing YU
;
Zhu-Lun ZHUANG
;
Hong-Xia LI
;
Yi ZENG
Author Information
- Publication Type:Journal Article
- MeSH: AIDS Vaccines; genetics; immunology; Adenoviridae; genetics; immunology; Animals; China; Genes, env; immunology; Genetic Vectors; genetics; immunology; HIV Antibodies; genetics; immunology; HIV Envelope Protein gp120; genetics; immunology; HIV Envelope Protein gp160; genetics; immunology; HIV-1; genetics; immunology; Mice; Mice, Inbred BALB C; Plasmids; genetics; immunology; Vaccines, DNA; genetics; immunology; Vaccines, Synthetic; genetics; immunology
- From: Chinese Journal of Experimental and Clinical Virology 2010;24(6):415-417
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct DNA and recombinant adenovirus vector vaccines containing an env gene from the prevalent subtype B strain in China and try to use them for therapeutic and prophylactic vaccines.
METHODSThe candidate plasmid DNA vaccine pVR-gp160 and recombinant adenovirus vaccine rAdV-gp160 were constructed separately. BALB/c mice were immunized with these two vaccines in different administration schemes. HIV-1 Gp120-specific cellular responses and antibody levels were detected by ELISPOT and ELISA respectively.
RESULTSDNA vaccine alone and combined vaccines in a DNA prime/rAdV-gp160 boost vaccination regimen induced high level of Gp120-specific cellular responses. While low level of Gp120-specific antibodies were elicited in all groups.
CONCLUSIONDNA and rAdV vaccines could efficiently express Gp160 protein and activate specific cellular responses.
