Immunogenicity of plasmid DNA and adenoviral vectors encoding HIV-1 subtype B env gene
10.3760/cma.j.issn.1003-9279.2010.06.007
- VernacularTitle:表达HIV-1 B亚型env基因的质粒DNA及腺病毒载体疫苗的免疫原性研究
- Author:
Hai-Ru YANG
1
;
Ling-Fei ZHANG
;
Xia FENG
;
Shuang-Qing YU
;
Zhu-Lun ZHUANG
;
Hong-Xia LI
;
Yi ZENG
Author Information
1. 中国疾病预防控制中心病毒病预防控制所
- Keywords:
AIDS vaccines;
Genes,env;
Plasmids;
Adenoviridae
- From:
Chinese Journal of Experimental and Clinical Virology
2010;24(6):415-417
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct DNA and recombinant adenovirus vector vaccines containing an env gene from the prevalent subtype B strain in China and try to use them for therapeutic and prophylactic vaccines. Methods The candidate plasmid DNA vaccine pVR-gp160 and recombinant adenovirus vaccine rAdV-gp160 were constructed separately. BALB/c mice were immunized with these two vaccines in different administration schemes. HIV-1 Gp120-specific cellular responses and antibody levels were detected by ELISPOT and ELISA respectively. Results DNA vaccine alone and combined vaccines in a DNA prime/rAdV-gp160 boost vaccination regimen induced high level of Gp120-specific cellular responses. While low level of Gp120-specific antibodies were elicited in all groups. Conclusion DNA and rAdV vaccines could efficiently express Gp160 protein and activate specific cellular responses.
