Application of SYBR Green real-time PCR assay for the specific detection of Salmonella spp.
- Author:
Seung Won SHIN
1
;
Seung Bin CHA
;
Won Jung LEE
;
Min Kyoung SHIN
;
Myunghwan JUNG
;
Anna YOO
;
Byeng Yeal JUNG
;
Han Sang YOO
Author Information
1. Department of Infectious Diseases, College of Veterinary Medicine and Brain Korea 21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Korea. yoohs@snu.ac.kr
- Publication Type:Original Article
- Keywords:
invA;
Salmonella spp;
specific detection;
SYBR Green real-time PCR
- MeSH:
Coat Protein Complex I;
Polymerase Chain Reaction;
Real-Time Polymerase Chain Reaction;
Salmonella;
Salmonella enteritidis;
Sensitivity and Specificity;
Stem Cells
- From:Korean Journal of Veterinary Research
2013;53(1):25-28
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The aim of this study was to applicate and evaluate a SYBR Green real-time PCR for the specific detection of Salmonella spp. Specificity of the PCR method was confirmed with 48 Salmonella spp. and 5 non-Salmonella strains using invA gene primer. The average threshold cycle (C(T)) of Salmonella spp. was 11.83 +/- 0.78 while non-Salmonella spp. was 30.86 +/- 1.19. Correlation coefficients of standard curves constructed using C(T) versus copy number of Salmonella Enteritidis ATCC 13076 showed good linearity (R2 = 0.993; slope = 3.563). Minimum level of detection with the method was > 10(2) colony forming units (CFU)/mL. These results suggested that the SYBR Green real-time PCR might be applicable for the specific detection of Salmonella spp. isolates.
