Interleukin-1β inhibits collagen synthesis and promotes its decomposition in cultured cardiac fibroblasts.
- Author:
Hua XIAO
1
;
Ai-Min JI
;
Zhi-Liang LI
;
Xu-Dong SONG
;
Dan SU
;
Ai-Hua CHEN
Author Information
1. Department of Cardiology, the Zhujiang Hospital, Nanfang Medical University, Guangzhou 510282, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cells, Cultured;
Collagen;
biosynthesis;
Fibroblasts;
cytology;
metabolism;
Interleukin-1beta;
pharmacology;
Matrix Metalloproteinase 2;
metabolism;
Matrix Metalloproteinase 9;
metabolism;
Myocardial Infarction;
Myocardium;
cytology;
Rats;
Rats, Sprague-Dawley;
Ventricular Remodeling
- From:
Acta Physiologica Sinica
2008;60(3):355-361
- CountryChina
- Language:English
-
Abstract:
The present study aimed to investigate the effects of interleukin-1β (IL-1β) at different doses on collagen synthesis and decomposition in cultured cardiac fibroblasts from neonatal Sprague-Dawley rat. Cardiac fibroblasts were treated with IL-1β (0.01, 0.1, 1, 10, 100 ng/mL) for 24 h. Cell DNA synthesis was measured by (3)H-thymidine ((3)H-TdR) incorporation and collagen synthesis was measured by (3)H-proline ((3)H-Pro) incorporation. Matrix metalloproteinase (MMP) activity was measured by gelatinase zymography. MMP-2, MMP-9 protein expressions were measured by Western blot. mRNA expressions of MMP-2 and MMP-9 were detected by reverse transcription-polymerase chain reaction (RT-PCR). Compared with that in the control group, the incorporation of (3)H-TdR and (3)H-Pro decreased in high-dose IL-1β groups (≥0.1 ng/mL) but not in low-dose IL-1β group (0.01 ng/mL). IL-1β significantly increased MMP-2 and MMP-9 activities. IL-1β (0.01-100 ng/mL) also dose-dependently increased the protein and mRNA expressions of MMP-2 and MMP-9 (P<0.05, P<0.01), respectively. These results suggest that IL-1β decreases collagen synthesis and MMP activities through transcriptional and posttranslational processes via degrading collagen in a dose-dependent way. Elevation of IL-1β is possibly involved in the process of ventricular remodeling after myocardial infarction, and the concentration of IL-1β is possibly a major factor which affects the extent of ventricular remodeling.
