Association of susceptibility to chronic rhinosinusitis with genetic polymorphisms of IL-4 and IL-10
10.3760/cma.j.issn.1673-0860.2012.03.008
- VernacularTitle:慢性鼻-鼻窦炎易感性与白细胞介素4和10基因多态性的关系
- Author:
Mei-li ZHANG
1
;
Pei-hua NI
;
Chang-ping CAI
;
Ni-jun CHEN
;
Shi-Li WANG
Author Information
1. 上海交通大学医学院附属瑞金医院
- Keywords:
Sinusitis;
Nasal polyps;
Interleukin-4;
Interleukin-1 0;
Polymorphism,single nucleotide;
Genetic predisposition to disease
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2012;47(3):212-217
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between the promoter polymorphism of IL-4 and IL-6 and chronic rhinosinusitis (CRS).Methods One hundred and twenty-three patients with CRS and 239 healthy controls in Shanghai region were chosen in this study.The genotype of IL-4 gene -33T ＞ C and -590C ＞ T were determined using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and the genotype of IL-10 gene -1082A ＞ G was determined using amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) method. Statistical calculations were performed using SAS 8.2 software.Results Significant differences were found in genotype distribution of-33T ＞ C and -590C ＞ T between the CRS group and the control group( x2 =6.6013,P =0.0102,x2 =6.6013,P =0.0304),and -33T ＞ C remained significant following application of the Bonferroni correction (P＜0.025).The relative risks of CRS with -33T ＞ C and -590C ＞ T were 1.818(P =0.0236,95％ CI 1.084 -3.050) and 1.838(P =0.0147,95％ CI 1.127 -2.997).There was linkage disequlibrium (LD)between the -33T ＞ C and -590C ＞ T.The coefficient of linkage disequlibrium ( D' ) was 0.77 and the related coefficient (r2 ) was 0.54.The -33T/-590T haplotype was associated with CRS and the relative risk was 1.653 (P =0.0130,95％ CI 1.107 -2.469).There were only two genotypes of IL-10 gene -1082A ＞ G and the frequencies of the AA and AG genotypes were not different between the CRS and control groups.Conclusion The promoter polymorphism of IL-4 -33T ＞ C and -590C ＞ T were associated with the susceptibility of CRS and the -33T/-590T haplotype was a risk facter for CRS,but there were no assiciation between the -1082A ＞ G and CRS.
